°ñ¼öÀ¯·¡Áٱ⼼Æ÷¿¡¼ ºÐÈµÈ °ñÀ¯»ç¼¼Æ÷¿¡¼ ¥â-TCP¿Í rhBMP-2ÀÇ °ñÇü¼º È¿°ú¿¡ °üÇÑ ¿¬±¸
The effects of ¥â-TCP/rhBMP-2 on bone formation in osteoblast-like cells induced from bone marrow-derived mesenchymal stem cells
ÃÖ¿ë¼ö, Ȳ°æ±Õ, ½É±¤¼·, ¹ÚâÁÖ, ÀÌÀç¼±,
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ÃÖ¿ë¼ö ( Choi Yong-Soo ) - ÇѾç´ëÇб³ ÀÇ°ú´ëÇÐ Ä¡°úÇб³½Ç ±¸°¾Ç¾È¸é¿Ü°ú
Ȳ°æ±Õ ( Hwang Kyung-Gyun ) - ÇѾç´ëÇб³ ÀÇ°ú´ëÇÐ Ä¡°úÇб³½Ç ±¸°¾È¸é¿Ü°ú
½É±¤¼· ( Shim Kwang-Sub ) - ÇѾç´ëÇб³ ÀÇ°ú´ëÇÐ Ä¡°úÇб³½Ç ±¸°¾È¸é¿Ü°ú
¹ÚâÁÖ ( Park Chang-Joo ) - ÇѾç´ëÇб³ ÀÇ°ú´ëÇÐ Ä¡°úÇб³½Ç ±¸°¾È¸é¿Ü°ú
ÀÌÀç¼± ( Lee Jae-Sun ) - ÇѾç´ëÇб³ ÀÇ°ú´ëÇÐ ÀÇ»ý¸í¿¬±¸¼Ò
KMID : 0355620080340040419
Abstract
The present study aimed to investigate the osteogenic potentials of differentiated osteoblast-like cells (DOCs) induced from bone marrow-derived mesenchymal stem cells (MSCs) on ¥â-tricalcium phosphate (¥â-TCP) with recombinant human bone morphogenetic protein (rhBMP-2) in vitro. Osteoblast differentiation was induced in confluent cultures by adding 100 nM dexamethasone, 10 mM ¥â-glycerophosphate, 50 mM L-ascorbic acid. The Alizarin red S staining and reverse transcriptase-polymerase chain reaction (RT-PCR) were perfomed to examine the mRNA expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), receptor activator for nuclear factor ¥êB ligand (RANKL), runt-related transcription factor 2 (RUNX2), collagen-¥° (COL-¥°). There were no significant differences in the osteogenic potentials of DOCs induced from MSCs on ¥â-TCP(+/-). According to the incubation period, there were significant increasing of Alizadin red S staining in the induction 3 weeks. The mRNA expression of ALP, RUNX2, and RANKL were higher in DOCs/¥â-TCP(-) than DOCs/¥â-TCP(+). According to rhBMP-2 concentrations, the mRNA expression of BSP was significantly increased in DOCs/¥â-TCP(+) compared to that of DOCs/¥â-TCP(-) on rhBMP 10 ng/ml. Our study presented the ¥â-TCP will have the possibility that calcium phosphate directly affect the osteoblastic differentiation of the bone marrowderived MSCs.
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mesenchymalstemcell;rhBMP-2;¥â-TCP;differentiatedosteoblast-likecell
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